![anemona nemorosa anemona nemorosa](https://www.mn.uio.no/ibv/tjenester/kunnskap/plantefys/botanikk/gulveis3.jpg)
nemorosa may have potential anti-proliferative properties. Tetramethylrhodamine ethyl ester staining showed a decrease in mitochondrial membrane potential at 24 and 48 h.
![anemona nemorosa anemona nemorosa](http://www.botanikaland.hu/botanica/japan-szellorozsa/5.jpg)
nemorosa treatment compared to the positive control after 24 and 48 h. Apoptosis was confirmed through fluorescent staining with annexin V-FITC. nemorosa yielded a delay in the early mitosis phase of the cell cycle. With a 50% inhibitory concentration (IC 50) of 20.33 ± 2.480 µg/mL, treatment with A. Cisplatin was used as a positive control. nemorosa extract by using HeLa cervical cancer cells. However, due to the reported abundance of saponins which usually exert anti-cancer activity via cell cycle arrest and the induction of apoptosis, we investigated the mode of cell death induced by an aqueous A. To date there has been no reported evidence of its use in the treatment of cancer. The Anemone plants have been found to contain various medicinal compounds with anti-cancer, immunomodulatory, anti-inflammatory, anti-oxidant and anti-microbial activities. Various parts of the plant have been used for the treatment of numerous medical conditions such as headaches, tertian agues, rheumatic gout, leprosy, lethargy, eye inflammation as well as malignant and corroding ulcers. The plant belongs to the regionally rare plants of Ukrainian administrative territories, therefore, despite the results of phytochemical and pharmacological studies, it becomes clear that the further use of Anemone nemorosa as a medicinal herb is an important issue for pharmacy and pharmaceutical biotechnology due to the prospects of research in area of new herbal remedies creation.ĭue to extensive experience of use in traditional medicine, a wide range of pharmacological activity, the content of biologically active compounds, Anemone nemorosa is a promising and valuable herbal material for phytochemical remedies production and their practical implementationĪnemone nemorosa is part of the Ranunculaceae genus Anemone (order Ranunculales) which comprises more than 150 species. The main biologically active substances of Anemone nemorosa are alkaloids, glycosides (Protoanemonin, Anemonin, Ranunculine, some types of saponins, tannins), vitamin C, resins, organic acids (chelidonic acid), coumarins, flavonoids and γ-linolenic acid. This herb is non-officinal, but it is widely used in traditional medicine as an antitumor, anti-inflammatory, antispasmodic, sedative, diaphoretic, bactericidal, antimicrobial, antifungal, expectorant, and diuretic agent. Anemone nemorosa is a perennial herb of the buttercup (Ranunculaceae) family. The aim of research is implementation of Anemone nemorosa in pharmaceutical and medical practice. Literature sources were analyzed and the data concerning the range, biologically active compounds content and the use in pharmacy and medicine of Anemone nemorosa were summarized.